Senecavirus A in Pigs, United States, 2015

completed sequencing the whole genomes for the vesicle tissue (SVA-OH1) and vesicle swab sample (SVA-OH2). On the basis of BLAST (http:blast.ncbi.nlm.nih.gov/ Blast.cgi) searches, the SVA-OH1 and-OH2 isolates had 99% nt identity to 3 new US strains The Ohio isolates also shared 96% and 94% nt identity with Canada strain (11-55910-3) and the first US SVA strain (SVV-001), respectively. Further analysis showed that, in comparison with these 8 strains with complete genome sequences available in GenBank, the 2 Ohio SVA isolates had 22 unique nucleotide mutations in the genome: 1 in the VP4 Among the 22 unique mutations, there were 2 nonsyn-onymous mutations at position 2082 in the VP3 gene of both isolates and position 5037 in the 3A gene of SVA-OH1 and 1 unique synonymous mutation only in SVA-OH2. Phylogenetic analysis of the complete genome further supports that the 2 Ohio SVA isolates are closely related to each other and clustered together with the 3 recently isolated US strains, were less closely related to the isolates of the Brazil cluster, and were more distantly related to the isolate from Canada and the original SVA strain reported from United States (Figure). Consistent with the previous findings (1), all SVA isolates from different countries clustered together under the genus Senecavirus, which is most closely related to the genus Cardiovirus of the family Pi-cornaviridae (Figure). Our findings that a pig with clinical signs of IVD was infected with SVA and our genetic analysis demonstrating that the 2 Ohio SVA isolates are closely related to the other SVA strains from different countries provide further support for SVA involvement in IVD in pigs. More support could be provided by future studies, including continued surveillance of SVA and confirmation of the Koch postulates. Acknowledgments We acknowledge and appreciate the excellent technical support provided by Jason Herr.